Datasets

All source data used in this study are made publicly available here. The released datasets include synthetic volumetric fluorescence imaging data, synthetic time-lapse calcium imaging data, and experimentally acquired light-sheet, widefield, and two-photon microscopy datasets covering subcellular structures, neuronal morphology and activity, calcium dynamics, and cell migration. We provide ~300 GB of data in total. All currently available data are listed in the table below and we have no restriction on data availability. You can download these data by clicking the DOI hyperlinks appended in the ‘Sample’ column. We recommend using ImageJ/Fiji to open and view these files.

No. Sample Events Imaging mode Pixel size Frame rate Imaging depth Data size Comments
1
Synthetic HeLa cells DOI
 Subcellular structures Confocal microscopy 0.032 μm/pxl – 0–4.096 μm (0.008 μm/plane) 7.9 GB Low-SNR / GT
2
Zebrafish GABAergic neurons DOI
 Neuronal structures Light-sheet microscopy 1.290 μm/pxl – 0–546 μm (1.50 μm/plane) 26.7 GB Low-SNR / high-SNR
3
Zebrafish GABAergic neurons DOI
 Calcium transient Light-sheet microscopy 0.160 μm/pxl 5 Hz – 92.4 GB Low-SNR
4
Freely behaving mice DOI
 Neural activity Head-mounted widefield microscopy 4.000 μm/pxl 30 Hz Layer 2/3 48.5 GB Low-SNR
5
Freely behaving mice DOI
 Behavior Overhead camera 0.400 mm/pxl 9 Hz – 0.2 GB –
6
Mouse dendritic spines DOI
 Calcium transient Two-photon microscopy 0.155 μm/pxl 30 Hz 40 μm 16.7 GB Low-SNR / high-SNR
7
Mouse brain neuropil DOI
 Calcium transient Two-photon microscopy 0.977 μm/pxl 30 Hz 40–180 μm 74.9 GB Low-SNR / high-SNR
8
Mouse brain neutrophils DOI
 Cell migration Two-photon microscopy 0.349 μm/pxl 10 Hz 30 μm 10.7 GB Low-SNR / high-SNR
9
Zebrafish multiple brain regions DOI
 Calcium transient Two-photon microscopy 0.873 μm/pxl 15 Hz – 10.4 GB Low-SNR / high-SNR
10
Synthetic cortical neurons DOI
 Calcium transient Two-photon microscopy 3.040 μm/pxl 50 Hz 200 μm 10.5 GB Low-SNR / GT